4.7.1. Micro-Manager¶

Preparation¶

• Install Micro-Manager. It is straightforward procedure and there are usually no problems with setup process.
• Download libximc-2.7.6 library distribution.
• Copy following DLLs from extracted directory ximc-2.7.6/ximc/win32/ to the Micro-Manager directory: libximc.dll, xiwrapper.dll, bindy.dll
• Install VC++ 2013 Redistributable Package from the extracted file ximc-2.7.6/ximc/win32/vcredist_x86.exe
• Connect a power supply to 8SMC4-USB and set the appropriate voltage for motors in your stage. Turn a power supply on.
• You need XiLab to check your 8SMC4-USB controllers and set it. XiLab version depends on the controller firmware version. You can download XiLab and update controller's firmware at this page: https://en.xisupport.com/projects/enxisupport/wiki/Software.
• Connect 8SMC4-USB controllers to computer via USB and open them in XiLab software. Click Restore from file... in Settings... window of XiLab and choose appropriate profile for your stage. Click Save to flash. For additional information visit: https://en.xisupport.com/projects/enxisupport/wiki/XILab_application_User's_guide.

Getting started with Micro-Manager¶

Run Micro-Manager¶

• Run Micro-Manager from the shortcut at your desktop or start ImageJ.exe application from installation directory. First time it will greet you and suggest to type some information about yourself.
• The next window contains drop-down list with configuration files. Choose None.

Configure hardware¶

• In the main window choose Tools → Hardware configuration wizard.

The process of hardware configuration consists of 6 steps:

1. At start, choose “Create new configuration” and click “Next”.

2. At the second step Micro-Manager prompts you to add hardware. If you want to use MicroManager with 8SMC4-USB motor controller choose Standa8SMC4 folder in down window with available devices. There are two drivers: Standa8SMC4Z for motor controller with one axis and Standa8SMC4XY for controller with 2 axes. When you select one of them, a dialog box with properties will appear.

3. In the Value column type the COM port number for motor controller in the next format:

xi-com:%5C%5C.%5CCOMn

Trailing letter n is a number of corresponding COM port (look at the screenshot above). You can find it in XiLab or in Device Manager → Ports. Click Ok. Unit Multiplier X/Y fields are leaved with default values. These values allow you to set stage speed.
4. For Standa8SMC4Z you should type COM port and Unit Multiplier too. Z axis is usually used as a focusing stage.
5. Camera is added in the same way. You need to know correct driver name for your camera. OpenCVGrabber driver may be used for any USB camera. Choose OpenCVGrabber folder in Available Devices. The next window will appear. Click Ok.

6. Click Next continue and Finish in the last step.

The system configuration is finished.

Device usage¶

We have installed and configured Micro-Manager and can check how does it work.

1. Set the pixel size parameter in Tools → Pixel Size Calibration.... There are you should type the pixel size value and mark parameters that Micro-Manager should consider. Click New to create new pixel size. In appeared window mark resolution of your camera (with OpenCVGrabber it is OpenCVGrabber - Resolution parameter) and type pixel size (in um). If you want to calculate pixel size parameter you need to know image size from microscope, its resolution and displacement (from screw pitch) for you stage.
2. Modify UnitMultiplierX and UnitMultiplierY (Tools → Hardware Configuration Wizard → select “Modify or explore the existing configuration” → Click “Next” → Double click to “Standa8SMC4XY” in Installed Device window). It is needed for correct conversion from stage movement distance to pixel size value change.
3. Choose Tools -> Stage Position List.... Click Set Origin to set zero of your stage coordinate system. Then, in the main window click Live. Choose Scrolling Tool (like a hand) in the ImageJ window. Make sure that cursor looks like a hand on the screen. Click the windows with the video from camera. Now you can control your stage by keyboard (arrow keys to control XY axis and U,J buttons to control Z axis).
4. You can scroll an image from a camera with the mouse cursor. Click Tools → Mouse Moves Stage (Use Hand Tool). Now click video window and drag it in the desired direction. Stage will move in the same direction. Double click will allow you to center the view on the chosen point in the image.